文章摘要
刘佳铭,李俊峰,梁生康,朱茂旭,李正嵩,杨燕群.应用煎炸废油发酵制备槐糖脂生物表面活性剂[J].精细化工,2018,35(6):0
应用煎炸废油发酵制备槐糖脂生物表面活性剂
Utilization of Waste Fried Oil for Production of Sophorolipid Biosurfactants by fermentation
投稿时间:2017-05-16  修订日期:2017-08-27
DOI:
中文关键词: 槐糖脂  假丝酵母菌  煎炸废油  发酵  条件优化
英文关键词: Sophorolipids  Candida bombicola  Waste fried oil (WFO)  Fermentation  Optimization of conditions
基金项目:国家自然科学基金(41371314);山东省科技发展计划项目(2015GSF121030);山东省重点研发计划(2017GHY15117);山东省高等学校科技计划项目(J14LE59)
作者单位E-mail
刘佳铭 中国海洋大学化学化工学院中国海洋大学海洋化学理论与工程技术教育部重点实验室 liujm_1123@126.com 
李俊峰 青岛科技大学海洋科学与生物工程学院  
梁生康 中国海洋大学化学化工学院中国海洋大学海洋化学理论与工程技术教育部重点实验室 liangsk@ouc.edu.cn 
朱茂旭 中国海洋大学化学化工学院中国海洋大学海洋化学理论与工程技术教育部重点实验室  
李正嵩 中国海洋大学化学化工学院中国海洋大学海洋化学理论与工程技术教育部重点实验室  
杨燕群 中国海洋大学化学化工学院中国海洋大学海洋化学理论与工程技术教育部重点实验室  
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中文摘要:
      应用正交实验,优化了假丝酵母菌O-13-1以煎炸废油为油溶性碳源发酵槐糖脂的条件,并应用高效液相色谱-高分辨串联质谱(HPLC-MS/MS)鉴定了所产槐糖脂同系物的组成及结构。结果表明,经正交实验优化的O-13-1菌株以煎炸废油为底物制备槐糖脂的摇瓶发酵条件为:摇床转速200r/min,双碳源添加量均为90g/L,温度为25℃,接种量为体积分数12%,槐糖脂产量在菌株稳定生长期的后期即204h时达到最大,为57.64g/L;O-13-1菌株以煎炸废油为底物所合成槐糖脂由9种乙酰基取代的内酯型和酸型槐糖脂同系物构成,内酯型槐糖脂同系物所占质量分数达76%。基于摇瓶得到的以煎炸废油为油溶性碳源的优化发酵条件,O-13-1菌株在5L自动发酵罐中转速500r/min时发酵合成槐糖脂产量可达163.28g/L。以煎炸废油为碳源发酵槐糖脂的原料成本较普通大豆油降低18%,在有效降低槐糖脂生产成本的同时,实现了煎炸废油的资源化利用。
英文摘要:
      The fermentation conditions of sophorolipids (SLs) by Candida bombicola O-13-1 with waste fried oil (WFO) as lipid substrates were optimized through orthogonal experiment, and the structures of individual SL components were quantified and identified by HPLC-MS/MS. The results showed that the optimized conditions in shake-flask were determined as follows: agitation 200r/min, carbon source added amount 90g/L, temperature 25℃, inoculum size 12% (volume fraction). The highest yield of SLs of 57.64g/L was obtained at the end of the stationary phase of yeast cell growth, i.e. 204h. The products were composed of 9 kinds of acetylated lactonized and acidic SLs, and the lactonic forms accounted for up to 76% (mass fraction). Moreover, the yield of SLs was as high as 163.28g/L in a 5L automatic fermentor under the optimized conditions of shake-flask with the exception of agitating of 500r/min. The material cost of SLs fermentation with WFO as carbon source reduced by 18% compared with soybean oil, and the resource utilization of WFO could be realized while effectively reducing the production cost of SLs.
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